Not so much on our ability to utilise fat - we do that all the time at rest! But on our propensity to store more fat - probably.
If you do long-duration cardio at an intensity that preferentially allows the energy to come from mainly - or exclusively - fat (the so-called 'fat-burning' zone) then you are sending a signal to your body that it should store more fat for such future exertions.
It sounds counter-intuitive because the opposite - short but intense bursts of activity (like HIIT) - uses much more glucose than fat but the by-product of anaerobic glycolysis builds up in the cytosol and continues to be cleared long after the exercise session has ended (the so-called 'after-burn' effect). Added to which, if you limit dietary carbohydrate (especially sugars), you have to replace any lost glycogen via gluconeogenesis, often using the lactic acid built up during the intense exercise as a substrate and fatty acids as a fuel for the gluconeogenic process. These are the reasons given for this type of short, intense burst/rest exercise being more effective for overall fat loss and conditioning than regular LISS cardio.
So why does the latter result in more fat loss while the former encourages more fat storage? Assuming the observation is correct, I'm not sure but I can take an educated guess - in the latter situation fatty acid oxidation follows glucose/glycogen usage and continues/ticks along 'in the background' for an extended period (some sources claim 48-72 hours), in much the same way as it would at rest anyway, while in the former case we never work up to an intensity that would greatly use or exhaust glucose/glycogen so immediately calls upon fatty acid oxidation as a direct fuel source for physical activity - thus creating a greater need for its replacement above and beyond what we may already carry.
This is my understanding of the research out there - either may be in error - but I hope it made some semblance of sense!


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I have some fairly good examples of real life people being in worse shape due countless extra hours of training each week, and have most of the science to back it up. 







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